A Semiconductor-Based Loc by Optical-Driven Nanoparticle Amplified Sensing Technology for Parkinson'S Disease Progression

A novel and unique Lab on a chip (LOC) is proposed for clinical applications in this proposal. An organic light-emitting diode (OLED) and light-addressable potentiometric sensor could be fabricated and integrated in the same chip by low temperature processes based on organic semiconductor substrate. With a smart integrated circuit (IC) to controlled the developed LOC, optoelectronic tweezer (OET) could be performed to manipulate magnetic beads with surface binding test of conventional antibody and aptamer. It could help to improve the limit of detection (LOD) of biomarkers for Parkinson’s disease (PD). The final target of LOD of α-synuclein, β-amyloid, ApoA1 and Tau is defined as 200 pg/ml, 400 pg/ml, 0.1μg/ml and 0.5 pg/ml , respectively. Clinical tests will be performed in the end of 3rd year to verify the results from plasma of PD patients. Two prototypes of organic semiconductor sensors and smart analysis system of Parkinson's syndrome could be demonstrated. They could help to early detection on the degradation of PD patients and following early treatments. Medical resource and living quality of patients could be improved. 1st Year/ Organic LAPS for α-synuclein peptide detection Section 1: ALD and Organic semiconductor applied in LAPS Section 2: Direct surface immobilization of antibody and antigen Section 3: Applied magnetic beads in microfluidics integrated with LAPS 2nd Year/ Optical-driven actuation to improve the limitation of Debye length of LAPS Section 4: High adsorption and high photocurrent of organic LAPS Section 5: ACEO operation for Debye length limitation improvement in organic LAPS Section 6: Surface modification on MB for 4 antibodies and peptide for LOD evaluation 3rd Year/Improve LOD of integrated LOC with 4 sensors Section 7: ALD Optimized organic LAPS on flexible substrate for reliability and lifetime Section 8: Actuating and sensing integrated chip for LOD test Section 9: LOD reduction & selectivity improvement for 4 target biomarkers

Project ID:PB10901-2637
External Project ID:MOST108-2628-E182-002-MY3