Andrographolide Restricted Inflammatory Response Contribute to Hepatic Cannabinoid Receptors Pathways and Lipogenesis in Mice with Steatohepatitis

Associated with a rising prevalence of obesity, cardiovascular diseases and hepatic steatosis is increasing in the world. We have engaged on the characteristics of various herbal medicines on hepatic disorder diseases for years, including hepatic fibrosis, apoptosis, and oxidative stress. Specifically, we focus on obesity-mediated hepatic oxidative stress, a model generated by high liquid diet administration for 15 weeks, have hypertriglyceridemia relative to normal diet mice. In addition, hepatic fat accumulation and cannabinoid receptors abnormal expression also involving in lipogenesis dysfunction in this model. In the objective of current project proposal, we will apply the proteomics techniques to perform the study of molecular mechanism of biologic functions of Andrographis paniculata (Burm.f.) Nees (Acanthaceae) a herbal plant that related to possible hepatoprotective responses and function. Therefore, the specific aims in this project are: Specific Aim I: We will clarify the activity of andrographolide to obesity or high fat diet-mediated aberrant hepatic function, including inflammatory cytokine gene expression; meanwhile, we also detect other potential factor(s) involved in cannabinoid receptors dysfunction. Specific Aim II: Roles of andrographolide on endocannabinoids receptors and the mechanisms of hepatic lipogenesis and steatosis in obesity mice. Specific Aim III: We will identify and study the “possible” compounds originated from Andrographis paniculata Nees responsible for lipogenesis and steatosis. In addition, we will investigate molecular mechanisms by possible andrographolide -mediated signaling in regulation of SREBP and PPAR family and fatty acid synthase. Specific Aim IV： The long-term objective of this project is to conduct of proteomics approach in understanding the activity of andrographolide and generate enough information to open the way to couple phytomedicine with genomic sciences.

Project ID:PC10108-0272
External Project ID:NSC101-2320-B182-018