Bu Zhong Yi Qi Tang (BZYQT) Inhibits Some Tumors Growth and Pilot Study to Assess the Mechanism of BZYQT in vitro Cell Lines and Fe-Qi Vacuity Mice Model

BU ZHONG YI QI TANG is known to increase the activity of NK and LAK cells. In a previous grant entitled ？HH？H？？BU ZHONG YI QI TANG (BZYQT) inhibits tumor growth,increases immunity and pilot clinical study to assess the efficacy of BZYQT in combination with biochemotherapy？HH？HH we have demonstrated its efficacy and safety in combination with biochemotherapy. Objective tumor response was observed on CT scan. Animal models to further study the combination of Chinese medicine and biochemotherapy are therefore very important. In this trial we will explore the effects of such combination both in vitro and in vivo. In this trial we will develop an animal model to study the effects of BZYQT including the morphological change of the tumor cells after BZYQT treatment, the growth curve of tumor cell lines after BZYQT treatment, the additive/synergistic effects of BZYQT on IL-2, and the induction of apoptosis and cell death by BZYQT. We will use cell lines developed in our hospital including melanoma cell lines MS-1 and ML-2, NPC cell lines, B16 cell line, RCC cell lines, lung cancer cell line Line-1 and thyroid cancer cell line. By screening these cell lines we will explore the effects of cell killing effects of BZYQT. Most of the animal models were established in immune-competent mice. The model does not necessarily fit the patients with stage IV malignancies. We will establish a Fe-Qi vacuity (FQV)？HH]？HH？HH？HH？HH？HH？HH？HH^ BALB/c mice model bearing line-1 lung cancer. By this model we will study the effects of BZYQT in vivo. Line-1 FQV mice will be divided into 3 groups: oral BZYQT, intraperitoneal BZYQT, and control group without BZYQT. BZYQT will be given on D7 of tumor inoculation. Four weeks after inoculation mice will be sacrificed. The metastases will be examined thoroughly to confirm the effects of BZYQT. The success of this project will confirm the effects of BZYQT, establish the animal model of FQV tumor-bearing mice, and lead to the internationalization of Chinese medicine.

Project ID:PG9305-0147
External Project ID:CCMP93-CT-107