Characterization of Flaviviral Ns1 Recombinant Proteins for Sensitivity and Specificity of New Developing Next Generation Poct Platform

Quick screening analysis of the new emerging viruses is one way to fight against these infectious diseases and is one of the current biomedical technologies to pursue the highest goal. In recent years, a new developed prototype machine, Blubox, was innovated by the cooperation of Blusense biotech company in Taiwan and the Danish University of Technology. The usage of nano-magnetic particles and optical laser biochemical detection technology, along with the low-cost Blu-ray reader and specially designed micro-channel discs, the development of detecting reader to the biological signal from clinical samples. In this proposal, in collaborating with the company's production and innovative Blubox reader, we want to accelerate this detection platform can be more quickly in the clinical practice of the application. The aims of this proposal are: (1) construct the flaviviral non-structural NS1 expression plasmids; (2) expression and purification of recombinant NS1 protein, the use of Blubox detection of antigenicity; (3) biochemical analyses of different flaviviral NS1 proteins; (4) antigenic cross-reactivity analysis of different flaviviral non-structural NS1 proteins and (5) characterization of extracellular NS1 proteins from flavivirus-infected cells. By conducting this proposal, we will assist the Blubox platform for pre-clinical molecular biology experiments and investigation, while simultaneously demonstrating and analyzing several different flavivirus NS1 proteins in the Blubox platform for cross-reactivity test. Finally, the platform will be suitable for the clinical application.

Project ID:PC10611-0011
External Project ID:MOST106-2622-B182-002-CC2