Chemical and Magnetic Interplay on the Endocytosis Receptor-Mediated Nanoparticle Internalization by Tumor Cells

Nanoparticles may serve as drug carriers that offer unique possibilities to overcome cellular barriers and facilitate cell labeling and targeted delivery. It has been well established that magnetic force enhances internalization of magnetic nanoparticles (MNPs) with superparamagnetic properties. In addition, some chemical reagents have been demonstrated to enhance internalization of nanoparticles, including poly(l-lysine) and tea catechins. Our previous work has demonstrated a synergistic effect of (-)-epigallocatechin-3- gallate (EGCG) and magnetic force on MNP internalization by glioma cells; however, the mechanism remains unknown. We plan to test a hypothesis that interaction of nanoparticles with specific receptors/proteoglycans on the cell surface and subsequent actin rearrangement is imperative for nanoparticle internalization, which is highly dependent on the chemical characteristics of the nanoparticle surface, whereas magnetic sensitivity for MNP internalization is dependent on the endocytosis receptor on the cell surface. Therefore, two models are proposed in the study: 1) Gallate derivatives, such as EGCG, may act on the laminin receptor 67LR and enhance MNP internalization in a magnetic force-sensitive manner; 2) highly positively charged polymers, such as poly(l-lysine), may interact with negatively charged heparin sulfate proteoglycan on the cell surface in a magnetic force-insensitive manner. Our study may promote understanding of nanoparticle-cell interfaces, and establish techniques for manipulation of MNPs in magnetic targeting.

Project ID:PC10608-1440
External Project ID:MOST106-2320-B182-002