Construction and Biomedical Application of a Three Dimensional Matrix with Reconstituted Collagen Fibrils in Anisotropic Orientation

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

The extracellular matrix (ECM) plays a critical role in the regulation of some important cellular processes such as maintaining differentiated cell phenotypes, wound healing, and vessel formation. Collagen is the major elements of the ECM and it also is the most abundant proteins in vertebral animal. Generally, collagen fibers are randomly distributed in most tissues, so that collagen fibers could balance the forces from any directions. But in tendons and ligaments, the collagen fibers arrange aligned to resist the forces from particular directions (anisotropy). Therefore, we propose utilized oriented scaffolds could guide cell growth along collagen fibers and promote osteoblast-like and Schwann cell to express specific cellular biological function. To date, there are many techniques to fabricate aligned collagen matrix, but those techniques almost destroy native structures of collagen. The aim of this project is to development a new technique to prepare a three dimension aligned collagen fibrils matrix without losing secondary structure of proteins by micro-reactors. And this fabrication process could entrap cells in the three-dimension aligned collagen fibrils matrix in one-pot process. We also would like to investigate the relationship between the cellular biological function expression and degree of reconstituted collagen fibrils alignment.

Project IDs

Project ID:PB10207-0371
External Project ID:NSC102-2221-E182-006
StatusFinished
Effective start/end date01/08/1331/07/14

Keywords

  • reconstituted collagen fibrils
  • three-dimension
  • anisotropy
  • D-periods

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