Project Details
Abstract
The extracellular matrix (ECM) plays a critical role in the regulation of some important
cellular processes such as maintaining differentiated cell phenotypes, wound healing, and
vessel formation. Collagen is the major elements of the ECM and it also is the most abundant
proteins in vertebral animal. Generally, collagen fibers are randomly distributed in most
tissues, so that collagen fibers could balance the forces from any directions. But in tendons
and ligaments, the collagen fibers arrange aligned to resist the forces from particular
directions (anisotropy). Therefore, we propose utilized oriented scaffolds could guide cell
growth along collagen fibers and promote osteoblast-like and Schwann cell to express specific
cellular biological function. To date, there are many techniques to fabricate aligned collagen
matrix, but those techniques almost destroy native structures of collagen. The aim of this
project is to development a new technique to prepare a three dimension aligned collagen
fibrils matrix without losing secondary structure of proteins by micro-reactors. And this
fabrication process could entrap cells in the three-dimension aligned collagen fibrils matrix in
one-pot process. We also would like to investigate the relationship between the cellular
biological function expression and degree of reconstituted collagen fibrils alignment.
Project IDs
Project ID:PB10207-0371
External Project ID:NSC102-2221-E182-006
External Project ID:NSC102-2221-E182-006
Status | Finished |
---|---|
Effective start/end date | 01/08/13 → 31/07/14 |
Keywords
- reconstituted collagen fibrils
- three-dimension
- anisotropy
- D-periods
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