Deep Red-Near Infrared Oled for Spr Biosensor Development to Perform Rapid and Sensitive Detection of Human Enteroviruses

In this research proposal, we projected a novel deep red-NIR organic light-emitting diode (OLED) light source for improvement of the performance of handheld optofluidic SPR biosensor. About the sensing configuration we proposed by using half cylindrical prism and flexible substrate OLED. The flexible substrate OLED can be bended and attached to the half cylindrical prism with the simple alignment of SPR sensor configuration. The proposed design has some advantages such as convergent incident angles of light by the prism geometry and spectrometer replaced by charge couple device (CCD) or CMOS image sensor. Therefore, bulky rotation motor is not required. We believe the sensing performance will be significantly improved compared with our recent portable platform. Moreover, the half cylindrical prism is made from plastic material, which reduces the net weight of the instrument, and potential for disposable component for infectious diseases sample. Nano pattern of sensing metal is also proposed to enhance the localized SPR for the extremely sensitive detection of the sensing. Several components of the platform will be produced in the collaboration with Taiwan companies, such as photodetector, OLED module, micro pump, and plastic prism for the effective and efficient component production. Consequently, the potential commercialization of the sensor platform can be expected as the research output target in the near future. The biology application in this proposed research is the detection of human enteroviruses in the complex medium such as, saliva prior to the detection of the clinical samples. This virus is reported associated to the encephalitis diseases caused by neurotropic viruses, in which its complicated infections by clinically is noted to be similar neuroinvasive disease such as aseptic meningitis and acute flaccid paralysis (AFP). We convince that the entity of these SPR biosensor systems allow the ease of diagnosis sensing of encephalitis disease to eliminate the drawbacks from the conventional methods. For example, ELISA and immunoblotting which is complex, non-real time, laborious, and time-consuming, although its method is powerful and accurate. Overall, SPR biosensor hold promising merits as rapid, low-cost, real-time, portable, low power and accurate sensing methods for the encephalitis disease detection based on EV71 viral infection.

Project ID:PB10608-3899
External Project ID:MOST106-2221-E182-041