Project Details
Abstract
Monoclonal antibodies are the fastest-growing drugs since they are the therapeutics. Chinese hamster cells
are the most widely used platform for protein drug production. Additionally, hybridoma technology in
monoclonal antibody production is the key platform for developing new protein drugs. However, several key
procedures such as cell line screening, culture optimizing processes, antibody analysis and recovery of
recombinant protein shall be developed in Taiwan. For example, the cell screening procedure does not have a
high efficient and low-cost platform now. The magnetic nanoparticles can be functionalized by modifying a
variety of ligands on surface. This research aims to develop multifunctional nanoparticles that can label on
cell membrane, capture the secreting protein, and have magnetic properties for cell screening. The
nanoparticles can isolate high-production cells at a low cost and provide efficient sorting from a population
of cells. Our objectives include the following topics: 1. The design of reactive functional group on the
nanoparticles such as hydroxyl, carboxyl and thiol groups. 2. The comparison of anchored ligands on cell
membranes such as cholesterol and long-chain fatty acids. 3. The crosslinking agent between the magnetic
nanoparticles and antibody such as carbodiimide, biotin-streptavidin and glutaraldehyde. 4. The effects of
type and length the spacer molecule such as polysaccharides and polyethylene glycol. 5. The conjugation
efficiency is evaluated by using glutaraldehyde, carbodiimide, biotin-streptavidin and other cross-linking
agents between magnetic nanoparticles and ligands. 6. The characterization of the multiple functions in
magnetic nanoparticles. Finally, the selection efficacy is evaluated by using the hybridoma model. This
project aims to provide the multifunctional nanoparticles as the platform for the screening
antibody-producing cells that will be useful for the pharmaceutical industry.
Project IDs
Project ID:PB10507-2470
External Project ID:MOST105-2221-E182-070
External Project ID:MOST105-2221-E182-070
Status | Finished |
---|---|
Effective start/end date | 01/08/16 → 31/07/17 |
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