Design of Magnetic Nanoparticles for Screening of Antibody Production Cell Lines

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

Monoclonal antibodies are the fastest-growing drugs since they are the therapeutics. Chinese hamster cells are the most widely used platform for protein drug production. Additionally, hybridoma technology in monoclonal antibody production is the key platform for developing new protein drugs. However, several key procedures such as cell line screening, culture optimizing processes, antibody analysis and recovery of recombinant protein shall be developed in Taiwan. For example, the cell screening procedure does not have a high efficient and low-cost platform now. The magnetic nanoparticles can be functionalized by modifying a variety of ligands on surface. This research aims to develop multifunctional nanoparticles that can label on cell membrane, capture the secreting protein, and have magnetic properties for cell screening. The nanoparticles can isolate high-production cells at a low cost and provide efficient sorting from a population of cells. Our objectives include the following topics: 1. The design of reactive functional group on the nanoparticles such as hydroxyl, carboxyl and thiol groups. 2. The comparison of anchored ligands on cell membranes such as cholesterol and long-chain fatty acids. 3. The crosslinking agent between the magnetic nanoparticles and antibody such as carbodiimide, biotin-streptavidin and glutaraldehyde. 4. The effects of type and length the spacer molecule such as polysaccharides and polyethylene glycol. 5. The conjugation efficiency is evaluated by using glutaraldehyde, carbodiimide, biotin-streptavidin and other cross-linking agents between magnetic nanoparticles and ligands. 6. The characterization of the multiple functions in magnetic nanoparticles. Finally, the selection efficacy is evaluated by using the hybridoma model. This project aims to provide the multifunctional nanoparticles as the platform for the screening antibody-producing cells that will be useful for the pharmaceutical industry.

Project IDs

Project ID:PB10507-2470
External Project ID:MOST105-2221-E182-070
StatusFinished
Effective start/end date01/08/1631/07/17

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