Development of Airlift/Stirred-Tank Combination Reactor(Ascr) Systems for Large Scale Production of Saussurea Involucrata Suspension Cells

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details


Recent development in plant tissue culture techniques of high value snow lotus medical plant has made it possible to use less differentiated callus to produce secondary metabolites. Fast growing suspension cells of snow lotus is applied in this project for scale-up of laboratory to commercial level of bioreactors is even more difficult for plant cells cultures than for most microbial fermentations. The main difficulties with scale-up systems are the shear stress sensitivity of the cells and mass transfer limitation. Therefore, low shear stress uniform mixing and sufficient oxygen transfer capacity are two essential prerequisites to the successful design of most plant cell cultural bioreactors. This proposal is a continuation of Ministry of Science and Technology Industry-Academia Cooperation Program project「Development and evaluation of snow lotus cell suspension for business applications」. In this study, a novel Airlift/Stirred-tank Combination Reactor(ASCR) is developed and designed for the efficient cell growth and production. The ASCR systems can proceed simultaneously mixing and aerating under various operating conditions. Therefore, it is expected that the novel bioreactor will provide high oxygen transfer rates to maintain suspended cells of snow lotus in a low-shear environment. Additionally, since the system can protect the suspended snow lotus cells in vigorously agitated culture medium in bioreactor, the production of secondary metabolites might be increased under the vigorous agitation. To optimize the system of snow lotus suspension cells, the composition of medium is also a condition that requires consideration. The aim of this study is to optimize the cultivation strategies for S snow lotus suspension cell and scale up with modified ASCR bioreactor.

Project IDs

Project ID:PA10901-0318
External Project ID:MOST108-2221-E182-040-MY2
Effective start/end date01/08/2031/07/21


  • snow lotus
  • Airlift/Stirred-tank Combination Reactor
  • suspension cell system
  • secondary metabolites
  • low shear sensitivity


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.