Development of Endoscopic Optical Theranostic System for the Study on in Vivo Tumor Discrimination and Photoablation

In this 3-year proposal, our goal is to develop an optical coherence tomography-guided laser surgery system, which can be integrated by the endoscope for in vivo diagnosis and therapy on tumorous tissue. Compared to other optical imaging techniques, OCT can provide the natures of label-free imaging, high-speed imaging (up to hundreds frames/s), higher imaging depth (＜ 3 mm), and higher resolutions (1-10 (am). In our previous study, OCT has been successfully used for clinical diagnosis of oral precancer. Additionally, laser surgery can induce photothermolysis, enabling to heat or vaporize biological tissue, which has become a common solution in clinic. Therefore,in this propose, our working items in each year are as follows: • The 1st year: (1) Develop nonlinear fiber laser to simultaneously generate a broadband light in 1260-nm spectral regime for OCT and a high-power pulsed laser at 1550 nm for laser surgery (2) Investigate photothermolysis on the animal model by using 1550-nm, high-power laser (3) Develop OCT-guided laser surgery system • The 2nd year: (1) Develop endoscopic OCT catheter and system (2) Develop functional imaging ability (angiography, blood velocity, etc.) and imaging processing algorithm (vascular density, vascular diameter distribution) (3) Use the developed endoscopic OCT system for in vivo scanning tumor model • The 3rd year: (1) Demonstrate automatic and accurate positioning algorithm for OCT guided laser surgery (2) Utilize laser ablation on hard tumor tissue and evaluate the outcome (3) Utilize laser ablation on vessels in soft tumor tissue and evaluate the outcome In the future, such optical theranostic system can be potentially used for various biomedical applications such as ENT, airway, esophagus, colon, and ophthalmology.

Project ID:PB10701-0272
External Project ID:MOST105-2221-E182-016-MY3