Development of High-Resolution and Fast System for Directional Neuronal Differentiation and Ion Actuation Quantification

A new high-resolution, high-speed and directional quantitative evaluation system for neuro cell differentiation and ion activity transportation is proposed in the project. A novel and compact methodology platform will be provided for neuron in vitro research by the integration of light-addressable potentiometric sensors, reflective digital microscope, and multi-wavelength laser source for the biomedical image with the direct monitor and measurement. Different wavelength laser source can be used to stimulate cell for the electrophysiological response. The directly culture, measure, spatial resolution of orientation, ion channel potential and its transmission speed can be obtained in this developed system for precision and rapid index in fundamental researches. The high efficiency and acceptable cost (Tool: 0.6M NTD & Chip: 1k NTD) of the final system could be expected to replace the conventional limited and expensive microelectrode array platform (Tool: 3M NTD & Chip: 1k NTD). The progress of project can be separated into 3 years with following items: 1st year: Development of the high-frequency LAPS Item 1: SOI-LAPS with wet etching process Item 2: HfO2 sensing membrane sputtered on IGZO by HIPIMS Item 3: Neuron cell directly cultured on sensing membrane 2nd year: Synchronization of optical and optoelectronic bio-imaging system Item 4: Quantitative analysis of limited cell number Item 5: The high-speed optical scanning module with dual wavelength Item 6: The optimization of high photocurrent and high-frequency LAPS 3rd year: Synchronization of optical and optoelectronic bio-imaging system Item 7: Microprinting and surface treatment on graphene to control the neuron differentiation Item 8: Light-stimulating neuron ion channel and electrical measurement Item 9: 2D dynamic imaging speed to 100μs/pixel

Project ID:PB10901-2064
External Project ID:MOST108-2221-E182-060-MY3