Development of Optical Coherece Microscopy for Application of Real-Time 3D Cell Image

  • Lee, Cheng-Kuang (PI)
  • Chiang, Chun Pin (CoPI)

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

Optical Coherence Microscopy (OCM) is a non-invasive real-time cell-level imaging technique based on optical coherence tomography (OCT) and confocal microscopy. OCM can provide real-time image with resolution of about 1~2 microns, which is comparable to general optical microscopy with resolution of about 0.2~2 microns. In clinical applications, biopsy is the diagnostic golden rule for cancer and precancerous conditions. Pathologists examine the histological architecture of the removed tissue’s cells from the suspected diseased region under an optical microscope. However the tissue preparative procedure including fixing, slicing, and staining consumes much time so that the real-time diagnosis is impossible. Generally OCT has the resolution of about 7~15 microns, which is not enough for cell imaging. OCM improves the resolution to 1~2 microns, hence the optical biopsy becomes possible. In this project, we will build up an OCM system and use the GPU technique to increase the image process speed for real-time imaging in the first year. And then an optical microscope system will be integrated with the OCM system for co-registered imaging in the second year to evaluate the consistency of the two imaging modalities. In the third year, we will develop a scanning head for clinical application

Project IDs

Project ID:PB10107-0944
External Project ID:NSC101-2218-E182-006
StatusFinished
Effective start/end date01/08/1231/07/13

Keywords

  • Optical Coherence Microscopy
  • optical biopsy
  • diagnosis for cancer and precancerous conditions

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