Development of Optical Coherence Microscopy for Real-Time Optical Biopsy Imaging.

  • Lee, Cheng-Kuang (PI)
  • Chiang, Chun Pin (CoPI)

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

Biopsy is the standard clinical cancer diagnosis method. Cancer-suspected tissue is removed by operation procedure and then the specimen is made through a series of processes including slicing, fixing, staining, impregnating, and so on. By microscopy, the pathologists observe the cell structure, cell distribution, or nucleus size of the specimen to judge the cancerous stage of the suspected tissue. However, biopsy has some unavoidable disadvantages. For example, tissue-removing causes the patients painful, error-sampling position would cause false-negative diagnosis, and the time-consuming specimen preparation processes make real-time diagnosis impossible. Optical Coherence Tomography, (OCT) is a real-time non-invasive imaging technique with resolution about 5~15 microns. It has been widely used in ophthalmology for retina or cornea structure imaging. The resolution of OCT is sufficient for imaging the tissue structure with scale about several tens to hundreds microns. However, OCT is hard to observe the cell structure or nucleus size with scale about 3~10 microns. This project will develop Optical Coherence Microscopy, (OCM) which retains the OCT advantages of high-speed and non-invasive characteristics and even improves the resolution up to 1~3 microns approaching the resolution of general microscope. Without the need of removing tissue from patients and the need of specimen preparation processes, OCM can obtain the biopsy images in vivo. Hence OCM can potentially realize "Optical Biopsy." In the on-going project started last year, we built up a basic OCM system. In the first year of this project, to achieve real-time imaging, we will accelerate image processing speed by using graphic processing unit (GPU). In the second year, we will integrate a microscope with our OCM system for co-registered imaging to verify the consistency between biopsy and OCM images. In the third year, we will develop a scanning probe for clinical trial.

Project IDs

Project ID:PB10207-1912
External Project ID:NSC102-2221-E182-047
StatusFinished
Effective start/end date01/08/1331/07/14

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