Development of Portable Nucleic Acid Amplification Test (Naat) Platform for Point-Of-Care Test (Poct)- Application of Nanoparticles with Photothermal and Magnetic Characteristics for Rapid and High-Performance Naat

Wu, Min-Hsien (PI)
Yang, Hung-Wei (CoPI)

Department of Biomedical Engineering

Project: National Science and Technology Council › National Science and Technology Council Academic Grants

Abstract

本研究計畫提出了一項光熱式珠粒上核酸增幅試驗技術，該技術係簡單地以一站式的方式，利用接合特殊抗體與引子且具有光熱及磁性的奈米粒子，來進行樣品前處理、光熱式珠粒上恆溫式圈環形核酸增幅、及後續利用側向流快篩試紙來進行檢測。在核酸增幅試驗中，與傳統的聚合酶連鎖反應技術或恆溫式圈環形核酸增幅技術相比，本項技術的優勢在於操作簡單、操作時間減少、且設備需求減少。最重要的是，它的檢測性能(尤其是檢測特異性）可以大幅提高。這些優勢使得該項技術成為即時檢驗應用的理想技術平台。整體而言、本項技術可應用於任何需要快速且精確的細菌/病毒/細胞檢測領域 (例如: 醫療、環保、食安、工業製程控制、犯罪偵查、或生物防恐)。

Project IDs

Project ID:PB11107-8309
External Project ID:MOST110-2221-E182-030-MY2

Status	Finished
Effective start/end date	01/08/22 → 31/07/23

Keywords

Nucleic acid amplification tests (NAATs)
Loop-mediated isothermal amplification (LAMP)
Point-of-care test (POCT)
Photothermal upon-bead NAAT (pubNAAT)
Polypyrrole-coated magnetic nanoparticles (PPy-MNPs)

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https://www.grb.gov.tw/search/planDetail?id=14511089

Development of Portable Nucleic Acid Amplification Test (Naat) Platform for Point-Of-Care Test (Poct)- Application of Nanoparticles with Photothermal and Magnetic Characteristics for Rapid and High-Performance Naat

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Abstract

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