Development of Protein Chips for Metal Phthalocyanine Dyes and Application to Early Diagnosis and Prognosis of Colorectal Cancer

With the progress of science and technology, human life expectancy has increased, but cancer mortality has increased year by year. In Taiwan, the number of colorectal cancer cases for the 11th consecutive year ranked first among the top 10 cancers and the 3rd death rate for cancer, and scientists are interested in early diagnosis of colorectal cancer. Early diagnosis and prognosis of colorectal cancer can be used to detect tumor-specific protein biomarker cancer embryonic antigens (Carciembryonicno antigen, CEA), and saccharides 19-9 (Carbohydrate antigen, CA19-9) to facilitate early diagnosis and prognosis of colorectal cancer. However, the specificity is not high. For this reason, the program plans to develop CEA, CA19-9, saccharides 72-4 (Carbohydrate Antigen, CA72-4) and vascular endothelial growth factor (vascular endothelialal) in order to improve the accuracy of early diagnosis and prognosis tracking of colorectal cancer. growth factor, VEGF) four protein sensing chips, with enzyme immunoanalysis (Enzyme-linked immunosorbent assay, ELISA) verification and cross-comparison, the proposed development with high sensitivity and single-selectivity, A colorectal cancer sensing chip with a short detection time.The program lasts for two years and includes: 1. Synthesis of metal phthalocyanine dyes with a three-dimensional structure as a development of metal phthalocyanine sensing chips with specific characteristics for CEA, CA19-9, CA72-4 and VEGF, respectively. 2. Preparation of magnetic nano- materials with nanomagnets as nuclei and metal phthalocyanine dyes as the shell, used to modify gold electrodes, and the use of electrochemical methods to develop a magnetic metal phthalocyanine sensing chips specific to CEA, CA19-9, CA72-4 and VEGF, respectively. 3. The above developed chips are used to detect the concentrations of CEA, CA19-9, CA72-4 and VEGF, respectively, and verify with ELISA results to define the best electrode conditions. 4. With the above-mentioned developed electrodes to detect the concentration of CEA, CA19-9, CA72-4 and VEGF in serum, only serum is used as the remaining detection of normal human and colorectal cancer patients. Cross-comparison and analysis of the above test results, and develop sensing chips that are stable for the environment, short detection time, high specificity and sensitivity, and can be applied to early diagnosis and post-treatment to track colorectal cancer.

Project ID:PB10907-4766
External Project ID:MOST109-2221-E182-012