Development of rapid detection kits for pathogenic bacteria by the combination of carbon quantum dots and isothermal amplification

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details


Traditional bacterial pathogen detection in the clinical laboratories requires 1-3 days’ culture and following identification steps, which is time consuming and expensive. The goal of this project proposal is to develop a rapid and simple method for detecting pathogenic bacteria in clinical samples, taking methicillin-resistant Staphylococcus aureus (MRSA) as an example. MRSA is resistant to many antibiotics and is a serious public health issue. The development of a rapid and simple test is important for MRSA Treatment and infection control. The test also has great market value. Loop-mediated isothermal amplification (LAMP) is an easy and efficient nucleic acid amplification method. However, the application of LAMP in MRSA detection encounters bottlenecks in nucleic acid purification and signal detection. This project proposal will solve these bottlenecks by combining LAMP with novel a bacterial lysis method and a signal detection method using carbon quantum dots (CQDs). The CQD formed by polyamines can easily lyse bacterial cell wall and expose the DNA when it is mixed with the bacteria. This method is much efficient than other methods for the preparation of bacterial DNA. Also, the CQD is compatible with LAMP, so the components can be premixed before the reaction. In addition, the platinum ion-modified spermidine CQD can enhance or quench the fluorescence from the amplified LAMP products. Therefore, this project will develop a ready-to-use kit for the detection of MRSA within 20-60 minutes. The device only requires a simple temperature controlling unit and a light source. Therefore, there are several advantages of our product, including less equipment requirement, simple procedures, and low costly reagents. In addition, the technology will be patented to protect out intelligent property. Therefore, it will be a competitive product on the market. The research groups of Chang Gung University, National Taiwan Ocean University, Chang Gung Memorial Hospital, and Bio-Helix company will apply this technologies to develop a series of rapid detection reagents for pathogens, which will be beneficial for solving important public health problems, assisting the growth of cooperative company, and promoting Taiwan’s technical capability.

Project IDs

Project ID:PA10911-0008
External Project ID:MOST109-2622-B182-003
Effective start/end date01/11/2031/10/21


  • rapid detection kit
  • methicillin-resistant Staphylococcus aureus
  • isothermal amplification
  • carbon quantum dot


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