Effect of Chronic Methamphetamine on Forebrain Serotonin Activity---Proteomic Study

Project: Ministry of Health and WelfareMinistry of Health and Welfare Commission Research

Project Details

Abstract

Amphetamine withdrawal usually accompanied with major depression, however, limited information regards its molecular mechanism are known. In this project, we will establish first the animal model of chronic methamphetamine (METH)-addicted rats by way of repetitive METH injections. Animals will be evaluated for its behavioral sensitization upon low dose METH challenge during withdrawal period. To assess their depressive behavior, animals will be explored to a novel environment during designated withdrawal days. The depressive animals will be subjected to biochemical analysis, such as serotonin transporter phorsphorylation, serotonin turnover, serotonin receptor characterization. In order to assess the intergrated aspect of METH addiction and depression, we will apply 2-D electrophoresis to investigate if particular protein species altered during behavioral depression stage. The overall data will allow us to understand the relationship between psychostimulant addiction and depression, and its molecular and cellular mechanism.

Project IDs

Project ID:PG9201-1071
External Project ID:DOH92-NNB-1019
StatusFinished
Effective start/end date01/01/0331/12/03

Keywords

  • methamphetamine
  • serotonin
  • drug addiction
  • prefrontal cortex
  • depression
  • proteomic
  • signal transduction

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