Effects of Hypoxic Exercise Training on Physiology by Metabolic Profiles in Human Biofluids_2

Exercise provides numerous salutary effects, such as to cut the risk of heart disease, improve in the delivery of oxygen to our body muscles, decrease the mental anxiety, and lower the blood pressure and cholesterol levels. Further research indicated that increased physical activity is associated with decrease incidence and mortality rates for various cancers. However, the media reported the injuries of top athletes, the potential for heat exhaustion and collapse by runners during races, which may be caused by elevated levels of oxidative stress, decrease fibrinolysis ability, and promoting platelet activation and coagulation cascades that damage the immune response. On the other hand, systemic hypoxia conditions occurring at high altitude lead to acclimatization processes in responses to minimize tissue damage, such as gradually increase pulmonary ventilation, enhance oxygen transportation efficiency in circulating system, and subsequently improve physical fitness. However, hypoxic states of human tissue belong to the most frequent and dangerous diseases of modern times. They results from disturbed oxygen supply to cells, which is insufficient to meet their metabolic demands. The beneficial or detrimental effects of hypoxia may vary substantially with the concentration of O2 exposure. Apparently not much work has explored how hypoxia/exercise intervention affects metabolic profiles of human biofluids, which could depend on the mode, strength, and duration of exercise, and will be the major topics of our serial research in this proposal. Metabolomics has been established as a powerful platform in biological sciences and found widespread applications in fundamental physiology, biomedical and environmental sciences. From our on going research, the recent study by LC-MS/MS presents a thorough profile of hypoxia’s or exercise’s impact on the human body’s metabolites in urine, and reveals vast biological differences with/without hypoxia or exercise intervention individually. We will continue this serial research and conduct a two-year work using metabolomics as the key platform technology. For the first year, the effects of urinary and plasma metabolic profiles by exercise integrating with hypoxia intervention will be determined. In addition, the changes of metabolites into the biological meaningful metabolic networking correlate to exercise integrating with hypoxia intervention will be identified and quantified as well. For the second year, following our previous results, the changes of metabolic profiles in response to proper long-term intermittent hypoxia/exercise training will be evaluated. The results obtained from these new and complex studies will develop suitable exercise combined environmental regimens for health-related sciences, and may further present a framework for assessing studies in exercise physiology. In addition, they will provide a better understanding of exercise physiology to guide individuals wishing to attain their objectives of optimal fitness and health with minimal risk, and update the knowledge base of exercise physiology.

Project ID:PC10007-1134
External Project ID:NSC100-2410-H182-018