Effects of Photodamages on Cellular Behaviors by Pulsed-Laser Actived Gold Nanoparticles

Engineered nanoparticles have been extensively used in versatile biomedical applications, especial for the plasmonic nanoparticles. These nanoparticles have demonstrated excellent optical scattering and photothermal properties in comparison to any molecular optical absorbers. After cell internalization of nanoparticles, laser-induced photothermal will cause the heating of the surrounding media, generated vapor bubbles etc. Although an increasing number of medical applications make use of laser-induced vapor bubble formation for damaging cell, the exact mechanism of when and how damage occurs remains unclear. Moreover, from the thermal response point of view, it is still unclear whether an intracellular delivery is better than an extracellular delivery due to conventional ideas of localization of heat by nanoparticles. Furthermore, the photothermal induced cellular damage is a transient or a permanent impairment of cells also unclear. In order to clarify the cellular response to the photothermal induced cellular damage, the proposal is aimed at examining the influences of photothermal induced damage to osteoblast-like cell (MG63) on the proliferation, differentiation, especial on the protein expression level by gold nanoparticles by under well-controlled pulsed laser conditions. Moreover, the study will also investigate the life-span of a single cell after laser-induced damages and reveal the photodamage potential role in nanoparticle mediated therapies and future biomedical applications.

Project ID:PB10308-2709
External Project ID:MOST103-2221-E182-012