Evaluation and Investigation of the Effect of Pl14736 on Reducing Restenosis and Its Underlying Mechanism

Since the first discovery of PL14736 from gastric juice in 1993, this pentadecapeptide has been found to efficiently enhance the healing of various tissues or organs. Nevertheless, the studies which have been conducted to explore the detailed molecular mechanisms are still very limited. Based on the studies of our laboratory in the past three years, we demonstrated the promoting effect of PL14736 on angiogenesis. We showed that PL14736 could accelerate the in vivo angiogenesis in CAM assay. In vitro study also found that PL14736 could enhance the migration and tube formation of vascular endothelial cells. Results from cDNA microarray confirmed the pro-angiogenic effect of PL14736. In addition, PL14736 could also promote the number and functions of endothelial progenitor cells and bone marrow-derived mesenchymal stem cells. We therefore proposed the hypothesis that PL14736 might accelerate the endothelialization of balloon-injured artery and reduce the chance of restenosis. We will evaluate and investigate the effect of PL14736 using the rat balloon injured carotid artery model in this two years project. Clinically, the chance of restenosis can be reduced by the use of drug-eluting stent. However, the long-term use of drug-eluting stent might increase the risk of thrombosis due to delayed endothelialization of the injured artery. This proposal aims to explore the beneficial effect of PL14736 which potentially can be applied to treat patients with high risk of restenosis or thrombosis in the future. As we know that the synthesis and purification of pentadecapeptide is relatively simple and small dose ranged between ng-μg/ml is already effective. The experimental results of this study will lead and shed light on the understanding of PL 14736 efficacy and explore the future application of PL 14736 therapy to related diseases.􀀡

Project ID:PC10108-0419
External Project ID:NSC101-2320-B182-042