Evaluation of p53 Mutations in Human Nasopharyngeal Carcinomas by Single Strand Conformation Polymorphism Analysis of Polymerization Chain Reaction Products

Chang, Yu-Sun (PI)

Molecular Medicine Research Center

Project: National Science and Technology Council › National Science and Technology Council Academic Grants

Abstract

p/sup 53/基因的變異是人類腫瘤中最常見的遺傳性變異,p/sup 53/蛋白為一核磷酸蛋白,共含 393個胺基酸,以前的報告指出p/sup 53/蛋白可與 SV40的T antigen以及人類乳突狀腫瘤病毒的E6蛋白形成一個結合體,此結合體可能與致癌之機轉有關.事實上,許多上皮細胞腫瘤組織中,均可以偵測到變異的p/sup53/基因及蛋白.鼻咽癌為上皮細胞腫瘤之一種,與EB病毒有密切的關係.同時約50%的腫瘤中亦有人類乳突腫瘤病毒的DNA(Chang et al., 1991).本研究計畫將以Polymerase chain reaction(PCR),Singlestrand conformation polymorphism(SSCP) 方法及免疫染色方法,來探討p/sup 53/基因及蛋白的變異,並評估其可能與EB病毒或人類乳突狀腫瘤病毒之關係.

Project IDs

Project ID:PB8303-1052
External Project ID:NSC83-0412-B182-008

Status	Finished
Effective start/end date	01/08/93 → 31/07/94

Keywords

Nasopharyngeal carcinoma
EB virus
HPV
p/sup 53/ mutation
PCR

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https://www.grb.gov.tw/search/planDetail?id=122142

Evaluation of p53 Mutations in Human Nasopharyngeal Carcinomas by Single Strand Conformation Polymorphism Analysis of Polymerization Chain Reaction Products

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