Explore the Pharmaceutical Effect and Associated Mechanism of Scutellaria baicalensis in DMN-Induced Liver Fibrotic Rat Model

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

Hepatic fibrosis is emerging as a treatable complication of advanced liver disease, following significant progress in understanding its underlying mechanisms. Scutellaria baicalensis Georgi is an important herbal plant widely cultivated and used in curing liver diseases. This genus has attracted great interest so much in the subjects of numerous chemical and clinical studies. Herein, we like to discover the effective products extracted from S. baicalensis Georgi for curing or protecting from hepatic fibrosis which is a pivotal step leading to liver cirrhosis or hepatoma in a DMN-induced liver fibrotic rat model. The hypothesis behind this project is to explore the effective components in S. baicalensis Georgi that may elicit specific cellular responses and signaling pathway to suppress and reverse liver fibrosis. This proposal will address the specific aims as follows: 1. In vivo screening: Using DMN-treated animal models to validate the function of effective components in treatment of hepatic fibrosis. At the same time, we try to explore the cytotoxicity, safety and drug targets through this experiment. Estimate the effect in suppression and reversal function in liver fibrosis induced by TGF-1 through MTT assays. Evaluate the cytotoxicity with LDH assays. 2. Using affymatrix oligonucleotide microarray, 2-DE analysis and MALDI-TOF/TOF mass spectrometry to connect intracellular networks and regulatory pathways of functional genes and proteins. Validate the obtained results by using IHC,IP,Western blotting and siRNAs experiments. 3. To systematically analyze the miRNA profiles and elucidate the regulatory effects on genes associated with hepatic fibrotic progression. 4. To establish the systematic biology of S. baicalensis Georgi through integrating information from genome, proteome, and metabolome. 5. Targeted therapy: apply the vitamin A-modified siRNA to inhibit the potential pathways or signaling cascades for uncovering the roles of certain genes or proteins for efficacy in liver fibrosis treatment. 6. Separation of organelles such as mitochondria to elucidate the association between oxidation stress and liver fibrosis. The value of study in S. baicalensis Georgi will contribute to our understanding of possible mechanism in anti-hepatic fibrosis. At the same time, the results should be helpful for clinical applications of Chinese medicine in future.

Project IDs

Project ID:PC10101-1653
External Project ID:NSC99-2320-B182-015-MY3
StatusFinished
Effective start/end date01/08/1231/07/13

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