Project Details
Abstract
Hepatic fibrosis is emerging as a treatable complication of advanced liver disease,
following significant progress in understanding its underlying mechanisms. Scutellaria
baicalensis Georgi is an important herbal plant widely cultivated and used in curing liver
diseases. This genus has attracted great interest so much in the subjects of numerous chemical
and clinical studies.
Herein, we like to discover the effective products extracted from S. baicalensis
Georgi for curing or protecting from hepatic fibrosis which is a pivotal step leading to liver
cirrhosis or hepatoma in a DMN-induced liver fibrotic rat model. The hypothesis behind this
project is to explore the effective components in S. baicalensis Georgi that may elicit specific
cellular responses and signaling pathway to suppress and reverse liver fibrosis.
This proposal will address the specific aims as follows:
1. In vivo screening: Using DMN-treated animal models to validate the function of
effective components in treatment of hepatic fibrosis. At the same time, we try to explore
the cytotoxicity, safety and drug targets through this experiment. Estimate the effect in
suppression and reversal function in liver fibrosis induced by TGF-1 through MTT
assays. Evaluate the cytotoxicity with LDH assays.
2. Using affymatrix oligonucleotide microarray, 2-DE analysis and MALDI-TOF/TOF
mass spectrometry to connect intracellular networks and regulatory pathways of
functional genes and proteins. Validate the obtained results by using IHC,IP,Western
blotting and siRNAs experiments.
3. To systematically analyze the miRNA profiles and elucidate the regulatory effects on
genes associated with hepatic fibrotic progression.
4. To establish the systematic biology of S. baicalensis Georgi through integrating
information from genome, proteome, and metabolome.
5. Targeted therapy: apply the vitamin A-modified siRNA to inhibit the potential pathways
or signaling cascades for uncovering the roles of certain genes or proteins for efficacy in
liver fibrosis treatment.
6. Separation of organelles such as mitochondria to elucidate the association between
oxidation stress and liver fibrosis.
The value of study in S. baicalensis Georgi will contribute to our understanding of possible
mechanism in anti-hepatic fibrosis. At the same time, the results should be helpful for clinical
applications of Chinese medicine in future.
Project IDs
Project ID:PC9907-2001
External Project ID:NSC99-2320-B182-015-MY3
External Project ID:NSC99-2320-B182-015-MY3
Status | Finished |
---|---|
Effective start/end date | 01/08/10 → 31/07/11 |
Keywords
- hepatic fibrosis
- Scutellaria baicalensis Georgi (SB)
- functional proteomics
- system biology
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