Fgf-Enriched Acellular Adipose Tissue Derived Hydrogel for the Promotion of Wound Healing in a Diabetic Animal Model

In Taiwan, the epidemiological studies revealed that the prevalence of diabetes mellitus during the past years was 12% and that the lifetime risk of a person with diabetes developing foot ulceration was reported as high as 25%. From the information of Bureau of National Health Insurance, there were NT $12,000,000,000 medical expenses for 600000 diabetic patients in 2006 in Taiwan. Poor wound healing in diabetes appears to be multifactorial, associated with growth factor deficiencies, impaired cellular function, microvascular disease, and dysregulated glucose control. Diabetic microangiopathy is characterized by abnormal growth and impaired microvasculature; it is attributed to a number of aberrantly expressed growth factors, possibly acting in combination to impaired wound healing. Treatment of chronic diabetic wounds remains one of the challenge issues for plastic surgeons regarding peripheral neuropathy and angiopathy. Tissue engineering covers the regenerative medicine by providing the concept of enhancing tissue regeneration with scaffold incorporating cells and growth factors to stimulate tissue growth. In our previous work, we have developed a technique on producing adipose-derived hydrogel and have proved it’s applicable in adipogenesis. Vascular formation was also identified in the same study. Furthermore, the effectiveness of the adipose-derived hydrogel might come from its enrichment of FGF as we have detected significant concentration of FGF in the previous study. The advantages of multi-potential bone marrow stem cells are including the differentiation into different target cell types, high concentration of cytokines and enhancing angiogenesis. These are the keys to promote wound healing in diabetic patients. In this proposal, we will try to incorporate our previous animal models, adipose-derived accelular hydrogel with and without stem cells to promote wound healing in diabetes mellitus type 2. The hydrogel derived from adipose or dermis is also compared in the promotion of wound healing in this diabetic animal model. In addition to the adipose-derived hydrogel, the FGF-enriched alginate may also accelerate the diabetic wound healing process.

Project ID:PC10101-1787
External Project ID:NSC99-2314-B182-015-MY3