Histomorphologic, Biomechanic, Transcriptomic and Proteomic Analyses on Guided Neo-Osteogenesis from Prefabricated Vascularized Periosteum--- in Vitro and in Vivo Animal Model

Background: The osteogenic property of combined vascularized periosteum grafting and pre-contoured biomaterials has not yet been well discussed and the mechanism needs to be elucidated. Our previous animal research regarding vascular periosteum grafting has documented the surgical techniques and osteogenic effects of vascular periosteum. In this study, we proposed a vascularized bone segment can be prefabricated from biomaterials in a physiological tissue chamber and then can be used for reconstruction of any bony defect. The osteogenic effects and angiogenesis mechanisms are analyzed via in vitro and in vivo studies. Materials and methods: Animal surgery (1st year): The osteogenic effect using a composite of vascularized periosteum and biomaterials is assessed in a soft tissue pocket using a rabbit tibia model. Replantation surgery of the prefabricated bone tissue and radiological studies are performed. Histomorphological analysis (2nd year): The healing process is assessed by histological examination, quantitative analysis and biomechanical test. Proteomics study (3rd year): Proteomics studies focusing on neo-osteogenesis and angiogenesis are conducted for the specimens of the composites. Assessment of Result: For animal model, histomorphological analysis of the histological sections by H-E stain, bone scintigraphy and regular x-ray examination of osteogenic repair are performed. In celluar analysis, gene transcription and proteomics studies are undertaken at different time period. Biomechanical compression is performed after replantation surgery. Statistics calculation is used for comparison among different groups. Prospect: Vascularized periosteum being a potent osteogenic and angiogenetic tissue can serve as an ideal grafting substitution of conventional bone graft. Not only can donar site morbidity can be minimized, but also the vascular periosteum can be pre-engineered before transplantation.

Project ID:PC10007-0363
External Project ID:NSC100-2314-B182-008