Influence of Whole Body Vibration on the Hormonal and Cytokines’Change in the Old Men

  • Wang, Shyi-Wu (PI)

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details


The aging people are increasing significantly in the world. It is estimated that the elderly people will be increased from 650 million at present to 2 billion in 2050. In Taiwan, according to the report from COUNCIL FOR ECONOMIC PLANNING AND DEVELOPMENT, the percentage of elderly people (>65 yr) will increased from 10.7 in 2010 to 41.6 in 2060. Also, the aging index will increased from 68.4% in 2010 to 441.8% in 2060. Therefore, the problems for these elderly people are: falls, frailty, decreased physical activity, and increased infection. Muscle weakness or sarcopenia, i.e. loss of skeletal muscle mass or muscle protein, is the major cause of frailty, subsequently falls, and death. How to enhance the muscle strength is an important issue for the elderly people at present. Testosterone or growth hormone supplements are often used in clinician, since these two hormones are considered to be the factors responsible for the synthesis and maintained of muscle protein. Studies, however, suggested that increased physical activity or exercise is more suitable and cheap method for elderly people to prevent muscle loss. Whole-body vibration is a gentle and suitable training for the elderly people, according to the study report. The present study is to investigate the influence of whole-body vibration training on biochemical parameter, immune and endocrine function by analysis the plasma cytokines(IL-1β, IL-2, IL-6, IL-15, and TNF-α), lactate, and hormones (e.g. testosterone, cortisol, growth hormone, insulin-like growth factors (IGF-I), ghrelin, and leptin) concentration. In addition, the effect of whole-body vibration training on the expression of IL-1β, TNF-α, IL-2, IL-6, and ghrelin mRNA from purified lymphocytes, monocytes, and neutrophils treated with PHA/PMA/LPS will be analyzed by reverse-transcriptase polymerase-chain reaction (RT-PCR). Our working hypothesis is that whole-body vibration training will regulate not only the plasma concentrations of cytokines and hormones but the mRNA expression in leukocytes in old men. Sixty to eighty elder men (>65 yr) will be randomly assigned to lying whole-body vibration (LWBV), standing whole-body vibration (SWBV) or control groups for 8 weeks. In addition, twenty young male (20-25 yr) will be as the control group to compare to the old groups. The blood samples will be collected before, during (4th wk), and after training. Plasma lactate, cytokines, and hormones will be measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of cytokines and ghrelin in leukocytes purified from the blood samples will be determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The specific aims for this study are: (1) to analyze the plasma concentrations of cytokines in control, lying, and standing group; (2) to investigate the expression of IL-6, IL-2, IL-1β, IL-15, and TNF-α mRNA in purified leukocytes. In the second year, we will investigate: (1) the plasma concentrations of ghrelin, leptin, testosterone, cortisol, growth hormone, IGF-I, and lactate; (2) the expression of ghrelin mRNA in purified leukocytes. All data will be expressed as mean ± SEM, and analyzed by the analysis of variance (ANOVA) and Duncan’s multiple range test. The correlation between cytokines in plasma, lactate, and hormones will be analyzed.

Project IDs

Project ID:PC10108-0485
External Project ID:NSC101-2410-H182-029
Effective start/end date01/08/1231/07/13


  • whole-body vibration
  • elderly training
  • hormone
  • cytokines
  • immunity
  • biochemical parameters


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