Influenza seed virus production by reverse genetics

Project: Ministry of Health and WelfareMinistry of Health and Welfare Commission Research

Project Details

Abstract

Emerging new strain of influenza virus has been posing a threat to humans. The mission of this project is to establish an experimental platform for generation of the vaccine seed virus from a newly emerging influenza virus. Viral hemagglutinin (HA) and neuraminidase (NA) genes will be cloned from the candidate strains provided by CDC. A recombinant influenza A virus by eight plasmids systems will be generated. Viral titer will be evaluated by plaque assay and its antigenicity will be measure by HI test. Four recombinant viruses are plan to be synthesized per year.

Project IDs

Project ID:PG9805-0220
External Project ID:DOH98-DC-1011
StatusFinished
Effective start/end date01/05/0931/12/09

Keywords

  • Reverse Genetics
  • Seed Virus

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