Project Details
Abstract
In Taiwan, the incidence of oral cancer has become the 6th leading cancer, the 4th
leading cancer in male in 2007, and is still increasing in the recent years. Since this
cancer usually occurs in the middle age male, at the high peak of life responsibility, it
has tremendous impact of family and society. Betel quid-chewing is the most common
habit in oral cancer patients of Taiwan, suggesting close association of this habit with
oral cancer formation.
Areca nut is the key element of betel quid, so that the carcinogenic effect may
mainly through the exposure of areca nut extract (ANE). Although accumulating
studies have reported the effects of areca nut on multiple cellular and molecular
alterations, the carcinogenic mechanism is still unclear. One reason is most studies
were examining the acute effects (short term and high dose) of ANE on oral cancer
cells, which is quite different from the actual chronic exposure of the risk.
Furthermore, there is no report of global gene expression survey in response to ANE
treatment.
In the present study, we consider actual carcinogenic process of area nut, mimic
chronic low dose (IC70) and long term (100 days) of ANE exposure, to treat and
establish sublines of oral mucosa cells. We then propose to investigate global
phenotype and transcriptome alterations, as well as the potential molecular signal
networking pathways by cDNA microarray and bioinformation analyses. The
functions of candidate genes and pathways in response to ANE will be further
validated through cellular and clinical based studies. Results of these studies will
provide valuable information on the carcinogenesis of areca nut-induced oral cancer.
Project IDs
Project ID:PC10001-0103
External Project ID:NSC98-2314-B182A-105-MY3
External Project ID:NSC98-2314-B182A-105-MY3
Status | Finished |
---|---|
Effective start/end date | 01/08/11 → 31/07/12 |
Keywords
- Oral cancer
- Areca nut extract (ANE)
- Transcriptome profiling
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