Integrating the Micrornaome into the Study of Enteroviruses Infection

Human enteroviruses (EV) and coxsackieviruses (CV) were the two major causative agents of hand-foot-and-mouth disease (HFMD) accounting for more than 70% of cases reported in recent outbreak in Taiwan. Understanding the host responses following enteroviruses infection should facilitate our understanding of viral pathogenesis and may accelerate our progress in the development of diagnostic and treatment strategies. Host microRNA (miRNA) regulates cell processes by inhibiting specific message RNAs via annealing to their 3’ untranslated region. Recently, miRNA has been reported playing an important role in the host cellular response to viral infections. In addition, altered circulating microRNA (miRNA) has been demonstrated that correlation with microbial infections in patients. The objective of this proposal is to provide the applicant with exemplary translational medical research training in the development of diagnostic biomarkers of enteroviruses infection related diseases as well as profiling the host genes involving in the viral infection cycle through the mircoRNA targets gene profile. There will be three specific aims listed as follow: (1) Profile the circulating miRNA from patients who were infected by enteroviruses; (2) Identify the miRNA that is uniquely expressed and studying how cellular genes are affected by the miRNAs after viral infection; (3) Investigate the miRNA in mice. We believe that this proposal represents a pilot investigation about the responses of host circulating miRNAs associated with enteroviruses infections

Project ID:PC10607-0345
External Project ID:MOST106-2320-B182-007