Investigation into the Mechanisms of Transplantation Toleterance Induced by Peptide Vaccines

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details


Naturally occurring CD4+CD25+ regulatory T (T-reg) cells not only play an important role in the maintenance of self-tolerance, but also control graft versus host disease and infectious disease, and prevent graft rejection. We have shown that the generation of adaptive CD4+ T-reg cells resulted from persistent but incomplete signaling in a mono-specific mouse and prevented allograft rejection. Antigen-reactive T-reg cells were also identified in the skin grafts. Female B6 mice are able to reject male B6 skin graft. In this project, we wish to further study the T cell regulation in this animal model. We wish to induce dominant transplantation tolerance to male skin graft with peptide-based vaccines in female B6 mice and to explore its underlying mechanism. Preliminary result showed that adoptive transfer of CD8+ T cell-depleted splenocytes could prevent male skin graft rejection by female mice. We will characterize the adaptive T-reg cells and study the extent of antigen specificity by testing linked suppression and infectious tolerance. To further understand the biology of the T-reg cells, we wish to explore the mode of action by the adaptive T-reg cells, including the interaction with dendritic cells, to demonstrate the distribution of T-reg cells and the role of IL-10 and IFN-γ in this peptide-induced tolerance. Finally, we study whether macrophage F4/80 receptor is required for the induction of antigen-specific CD4+ T-reg cells in this animal model. If we can realize the underlying mechanisms of transplantation tolerance, then we might be able to harness them for therapeutic benefit.

Project IDs

Project ID:PC9706-0163
External Project ID:NSC95-2320-B182-042-MY3
Effective start/end date01/08/0831/07/09


  • transplantation tolerance
  • regulatory T cell
  • peptide vaccine


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