Nano-Gold Composite Modified Polycrystalline-Silicon Nanowire Field-Effect Transistor for Early Cancer Diagnosis for Early Cancer Diagnosis

In the development of biosensors, the utility, reliability, and stability of the working electrodes are the most important key factors that should be considered. Meanwhile, the biosensors must have a high sensitivity, fast response time, good selectivity, and low cost to meet the requirements for practical applications. In Taiwan, prostate cancer (PCa) and bladder cancer are the fifth and ninth most common cancers in men. In addition, the numbers of patients increase with the increasing of years. In order to inprove the accuracy of the early-stage detection of PCa and bladder cancers, a platform with good stability, high accuracy and fast response time for the detection of those cancers is proposed in this project. This is a three-year project. Materials such as nano-size gold (nano-Au), magnetic nano-size gold (nano-Fe3O4@Au), nano-rod gold with porous silicon dioxide (nano-SiO2@AuR), and magnetic nano-rod gold with porous silicon dioxide (nano-SiO2@Fe3O4@AuR and nano-SiO2@Ni@AuR) are synthesized and prepared. Those materials are used as carriers for prostate-specific membrane antigen (PSMA), prostate-specific membrane antibody (PSMAab), and apolipoprotein A1 protein antibody (anti-AOPA1). The effective immobilization degrees and bioactivities of PSMA (a biomarker for PCa) and anti-APOA1 (a biomarker for bladder cancer) on the nano-carriers are verified by cell experiments. A platform for the detection of PSMA and anti-APOA1 is developed based on an n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). The concentrations of PSMA and anti-APOA1 captured by the nano-carriers are analyzed by poly-SiNW-FET with and without the applied magnetic field. Furthermore, the enzyme-free mechanism for the detecting hydrogen peroxide proposed by our laboratory is applied for the detection of PCa and bladder cancers. The concentrations of hydrogen peroxide from the patient’s urea are detected by electrochemical process under the negative applied voltages. The correlations between the concentrations of hydrogen peroxide and PSMA and anti-APOA1 are investigated

Project ID:PB10501-3840
External Project ID:MOST104-2221-E182-077-MY3