Poly(O-Aminobenzoic Acid) Modified Gold Electrode for Early Cancer Diagnosis for Early

With the prolongation of human lifespan and the increase in cancer mortality, scientists have an intense interest in early-phase diagnosis of cancer and in improving the survival rate for cancer patients. A promising way to realize early diagnosis of cancer is to detect the cancer-specific protein biomarkers. In the development of biosensors, the practicability, convenience and stability of the sensing electrodes are important and must be considered, in addition, for the object to be tested with high sensitivity, short detection time, specificity selectivity and cheap, etc. is the evaluation of the sensor has no practical reference pointers. This is a three-year project. To improve the accuracy of early diagnosis of lung cancer, breast cancer and prostate cancer, the program includes: 1. Nucleic acid apatmer (AP) with specificity of EGFR is proposed. 2. Synthesis of 3D nano-poly(o-amino benzoic acid) (PAnCOOH) with modified gold electrode. 3. The PAnCOOH are connected with aptamer by nucleic acid, CRP antibody (CRPab), VEGF antibody (VEGFab), EGFR antibody (EGFRab) or PSMA antibody (PSMAab), respectively, using cell experiment and ELISA to verify the above vectors can effectively catch CRP, EGFR, VEGF and PSMA. 4. The modified electrodes were used to detect the CRP, EGFR, VEGF and PSMA concentrations in the samples by electrochemical method. 5. The use of multiple certification to improve the accuracy of early diagnosis of cancer, is expected to develop a stable environment, cheap, fast, specificity and high sensitivity, commercial cancer electrochemical measurement platform.

Project ID:PB10707-0402
External Project ID:MOST107-2221-E182-019