Project Details
Abstract
Tumor growth, invasion, and metastasis are a complex process that includes cell division
and proliferation, proteolytic digestion of the extracellular matrix, cell migration through
basement membranes to reach the circulatory system, and remigration and growth of tumors
at the metastatic sites. F-18 FDG PET has been widely used for clinical localization of tumor
lesions, however, it provides few information about the capability of distant metastases of the
malignancies.
The migration and metastasis of tumor cells require gelatinase proteolytic function of
MMP to degrade the basement membrane and extracellular matrix. The expression of MMP in
many tumors is significant higher than normal. Therefore, the distribution of MMP may
become a valuable biomarker to evaluate the capability of distant metastasis of the tumor
cells.
A cyclic peptide, CTTHWGFTLC (CTT), was reported as an effective MPP gelatinase
inhibitor for tumor therapy. The aims of this project are to develop a CTT-based
DOTA-peptide conjugate labeled with Ga-68 for PET imaging, and to evaluate the
characterization of this new tracer in tumor-bearing animal models.
In year 2010, we have synthesized DOTA-CTT peptide conjugate and verified its quality
with HPLC and mass spectroscopy. Ga-67-labeled DOTA-CTT peptide conjugate has been
achieved with high radiochemical purity.
In this project, the mouse models respectively bearing MDA-MB-231 and
MDA-MB-435S cells, two human breast tumor cell lines expressing different levels of MMP,
will be established. The in-vivo distribution of Ga-67/Ga-68-DOTA-CTT will be evaluated in
both animal models by dissection and animal imaging.
Project IDs
Project ID:PB10101-3747
External Project ID:NSC101-2623-E182-001-NU
External Project ID:NSC101-2623-E182-001-NU
Status | Finished |
---|---|
Effective start/end date | 01/01/12 → 31/12/12 |
Keywords
- radiogallium-labeld tracer
- peptidic gelatinase inhibitor
- tumor imaging agent
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