Preparation of Carbon Nanomaterials for the Application of Chemiluminescence Reagents

Chemiluminescence (CL) is a commonly used detection technique in biology and is usually catalyzed by horseradish peroxidase (HRP) conjugated antibodies. In chemiluminescence, energy is released by a chemical reaction, which triggers light generation. In recent year chemiluminescence has been widely used in environmental monitoring, clinical diagnosis and food safety due to its highly sensitive, fast and simple nature. However, chemiluminescence suffers from weak signals, short luminescence time, low intensity and a lack of reproducibility due to its rapid reaction. In order to solve these problems, enhancers were developed and added to the original chemiluminescence system, also known as, enhanced chemiluminescence (ECL) system. Compared with the conventional chemiluminescence, enhancers can easily react with HRP-I (oxidized HRP intermediate I, HRP-I) to increase the yield of free radicals; therefore, is more effective to increase the luminous efficiency. However, the enhancement efficiency of traditional enhancers still has room for improvement. Therefore, the purpose of this project is to develop a new enhancer based on carbon nanomaterials and apply them to chemiluminescence system. The carbon nanomaterials (CNMs) are synthesized by calcination of phenolic molecules and halide ions. This method can be used to synthesize CNMs with different functional groups on the surface. The HRP-catalyzed chemiluminescent oxidation of luminol can be used in many bioassays, such as western blots and enzyme-linked immunosorbent assays. We expect to develop CNMs-based enhancers which not only have the usual function of the enhancer, but also participate in the unique catalytic reaction of the chemiluminescence reaction. CNMs’ large surface area can increase the reaction rate and improve the electron transfer, achieving higher sensitivity for the detection.

Project ID:PC10706-0011
External Project ID:MOST107-2622-B182-001-CC2