Rapid Expression System for Human Embryonic Kidney Cells

The development of Proteomics and Genomics makes recombinant proteins as important drug targets. At the initial stage of a preclinical trial, several mini-grams of recombinant protein drug is needed for test. A rapid recombinant protein expression is very important for these purposes. HEK 293 cells combined with nonviral transfection techniques and different gene targets can produce different humanized protein at the same utility at a short interval. This project will take two years to develop the HEK recombinant protein expression techniques. In the first year, the plasmid (GFP, green fluorescence protein) production and purification procedure, three protocols for plasmid DNA transfer into HEK, and effects of chemical reagents on plasmid DNA transfer into HEK will be studied and optimized. In the second year, we will clone the human M-CSF gene from mesenchymal stem cells and transfect the gene into the HEK 293 cells using the developed protocol. The M-CSF from HEK293 will be compared with that from CHO cells to evaluate their glycosylation patterns by 2D-PAGE/MALDI.

Project ID:PB9408-4073
External Project ID:NSC94-2214-E182-008