Screening of Plant Lipases and Applications to Biodiesel Production

Lipases have been proposed as the biocatalyst to synthesize biodiesel, as the enzymatic process can avoid above shortages to be regarded as a substituent of the alkaline process. However, the high enzyme cost and lower transesterification reactivity has hampered its application in industry. This project is aimed to improve the shortages of the enzymatic process. The strategy includes: screening a versatile and recoverable lipase from various plant latex that is cheaper with higher activity, the best reaction condition. In order to meet the requirement of the domestic biodiesel plants, we will integrate the enzymatic process as the second-stage transesterification of the alkaline process, where the substrate and the product purities are set at 95% and 99%. Moreover, the hydrolysis of oils and fats as well as the esterification of fatty acids will be carried for comparing the performance from different lipases.

Project ID:PW9611-1085
External Project ID:96農科-1.2.1-科-a2(25)