Study for preparation of 99mTc-ubiquicidin 29-41 and its application to detect bacterial infections

The discrimination between bacterial infection and sterile inflammation has been a dilemma often faced by medical community. 67Ga-citrate has long been the tracer for practice of scintigraphic detection of inflammation in vivo, however, it takes 24-48 hours for systemic 67Ga clearance before image acquisition, and is unable to discriminate between infections and sterile inflammatory processes. Among many antimicrobial peptides investigated, a fragment of human macrophage peptide, ubiquicidin (UBI) 29-41, has been reported to be labeled with 99mTc, the most widely used radionuclide in nuclear medicine. The objectives of this project include: (1) to develop a kit formulation for preparation of 99mTc-labeled UBI 29-41; (2) to establish biokinetic information of 99mTc-UBI 29-41 in healthy experimental animals; (3) to establish an animal model of bacterial infection; (4) to establish biokinetic information of 99mTc-UBI 29-41 in the animal model of bacterial infection; (5) in-vivo 99mTc-UBI 29-41 imaging in the animal model of bacterial infection.

Project ID:PG9606-0043
External Project ID:962001INER050