Study of the Effects of Opticle Torque Induced by Laser-Actived Nanowires on the Fibrils Structures of Reconstituted Collagen and Biomedical Engineering Application

To date, researchers typically incubated cells with randomly assembled collagen fibrils matrices. Although promising results have obtained for the interest tissues which possess a lower organized level of ECM, there has been limited success in tissues comprise highly-organized ECMs. Several approaches have been introduced to produce aligned collagen matrices in vitro such as electro-spinning, magnetic fields, microfluidic flow, spin-coating etc. However, most of these techniques cannot create three-dimensional well-aligned "native" collagen fibril matrices containing embedded cells. Meanwhile, numerous studies have demonstrated using a laser light can induce alignment, rotation, and spinning of plasmonic nanoparticles because of the high long-axis dipole polarizability. Therefore, this study will focus to develop a new process fabrication an anisotropy, three-dimensional, and preserving the D-period of collagen matrix by optical torque induced by laser-activated gold nanorods/nanowires. And during the fabrication process could entrap cells within the three-dimension aligned collagen fibrils matrix. We expect the new fabrication system not only can overcome the general problems of layer-by-layer process, but also can introduce the optical force to the application of 3D printing scaffolds. To address the application of the new system, the present proposal will use Schwann cells and osteoblast-like cells, MG63, as model to investigate the relationship between the parameters of fabrication process and the cellular biological function expression.

Project ID:PB10507-2924
External Project ID:MOST105-2221-E182-007