Project Details
Abstract
Flaviviruses consist of viruses causing yellow fever virus, dengue fever virus, Japanese
encephalitis virus, andWest Nile. In mammalian cells, the entry of flaviviruses is generally
initiated by binding of the viral envelope protein with receptors or co-receptors such as
heparan sulfate distributed on the cell surface, followed by V-ATPase-dependent
clathrin-mediated endocytosis. However, it remains to be confirmed how flaviviruses infects
cells of the mosquito that are alternate host in nature. Usually, arboviruses cause trivial
deleterious effects to infected mosquito cells but induce apoptosis in vertebrate cells,
suggesting that host-virus interactions are relatively distinct in different combinations of
viruses and host cells. Apparently, host factors probably participate in most steps of virus
infection. In this study, genes up-regulated in C6/36 cells in response to flaviviruses infection
will be identified via the technique of subtractive hybridization. In our preliminary results, at
least 3 up-regulated genes have been identified, including V-ATPase (a proton pump), C189 (a
tetraspanin), and eIF5A (eukaryotic initiation factor 5A). Each of them is now known possibly
involved in various cellular functions according to results from the infection of other viruses.
Hypothetically, more novel and important genes involving in flavivirus infection is
anticipated to be identified; from which a web to elucidate interactions between flaviviruses
and mosquito cells may be established through this study. For genes presumably involved in
the infection will be identified and selected to elucidate their function via techniques such as
RNA interference. In addition, genes with important function will be sequenced and cloned
for protein expression. As well, these genes will be used to study interactions between the
virus and the host cell during/after infection.
Project IDs
Project ID:PD9706-0234
External Project ID:NSC96-2628-B182-003-MY3
External Project ID:NSC96-2628-B182-003-MY3
Status | Finished |
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Effective start/end date | 01/08/08 → 31/07/09 |
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