Study the Intracellular Inhibited Signaling Controlled on Human CD4+T Lymphocytes in the Patients of Perennial Allergic Rhinitis Treatment by Mixed Formula of Chinese Medicine

The prevalence rate of allergic rhinitis gradually increased in recent years. Chinese traditional herbs have been tried in the treatment of allergic diseases by many authors, like Kaneko et al. 1997. However, these authors only used one compound formula in their studies. In our previous studies, a new mixed formula of Chinese herbs was first used to treated the patients suffered perennial allergic rhinitis, from the results of study, we believe the unusual modulation of Th1/Th2 cytokine in mononuclear cells and suppressed COX-2 expression in PMN become the immunological mechanism of mixed Chinese herb formula therapy in the suppression of allergic rhinitis. Further study, we found in high IgE group, the cytokines IL-10 and IL-12 secretion from DCs were elevated after treated but we did not found same condition in lower IgE group, the expression of HLADR; co-stimulators CD54 and CD 86 were suppressed after treated with herbal medicine for 3 months, the cytokines of T-helper cells seems shift to Th1 activation after herbal medicine treatment, so we get the conclusion that Chinese herbs can modulate the immunologic function in the treatment of allergic rhinitis via regulated the DCs functions. From our previous studies we found the function of Treg in allergic patients were lower than normal individuals, but the expression of CTLA4 and FOXP3 will enhanced after treated by Chinese medicine, it means that Chinese medicine treatment can modulated the functional activity of Treg. Further evaluated the cytokines change of IL-6; IL-17 and IL-23 by real time PCR, we found the expression of IL-17 and IL-23 were attenuated after Chinese medicine treatment, this result compatible with our hypothesis that Chinese medicine play an role of anti-inflammation in the treatment of allergic rhinitis. The study of this year, we investigate the inhibited signal transduction of CD+4 Tcells. We found the expression of SOCS1 and SOCS3 were attenuated after treatment by Chinese medicine, these results compatible with our previous studies that the treatment of Chinese medicine could modulated the function of Th2. In the next two years, will investigate the protein contains of STAT1; STAT3; STAT4; STAT5; STAT6; psSTAT1; psSTAT3; psSTAT4; psSTAT5; psSTAT6; JAK2; psJAK2 and gene expression of SOSC1; SOSC3; SOSC5; GATA3; T-bet in CD+4 T cells before and after treatment of Chinese medicine, these results will help us to realize the immune-modulation effects of Chinese medicine on the treatment of allergic rhinitis.

Project ID:PC10301-0307
External Project ID:NSC102-2320-B182-017-MY2