Study the Proteins Contains of Nasal Discharge and Intracellular Signaling Controlled on Human RCD4+ T Cells in the Patients of Perennial Allergic Rhinitis Treatment by Mixed Formula of Chinese Medicine

The prevalence rate of allergic rhinitis gradually increased in recent years. Chinese traditional herbs have been tried in the treatment of allergic diseases by many authors, like Kaneko et al. 1997. However, these authors only used one compound formula in their studies. In our previous studies, a new mixed formula of Chinese herbs was first used to treated the patients suffered perennial allergic rhinitis, from the results of study, we believe the unusual modulation of Th1/Th2 cytokine in mononuclear cells and suppressed COX-2 expression in PMN become the immunological mechanism of mixed Chinese herb formula therapy in the suppression of allergic rhinitis. Further study, we found in high IgE group, the cytokines IL-10 and IL-12 secretion from DCs were elevated after treated but we did not found same condition in lower IgE group, the expression of HLADR; co-stimulators CD54 and CD 86 were suppressed after treated with herbal medicine for 3 months, the cytokines of T-helper cells seems shift to Th1 activation after herbal medicine treatment, so we get the conclusion that Chinese herbs can modulate the immunologic function in the treatment of allergic rhinitis via regulated the DCs functions. The regulatory T cell response is characterized by abolished allergen-induced specific T-cell proliferation and suppressed T helper 1 and Th2-type cytokine secretion. The increased levels of interleukin-10(IL-10) and transforming growth factor-that are produced by Treg cells, potently suppress IgE production, as our previous studies, we found IL-10 production modified by the treatment of Chinese herbal medicine play an important role on the immuno-modulatory effects of Chinese herbal medicine. From our previous studies, some unknown protein in nasal discharge can induced and enhanced the inflammatory reaction. In this study, we try to find out the protein in nasal discharge that can modulate the allergic reaction by the method of western blotting. Besides, we also try to evaluate and assay the concentrations of microRNA and suppressor of cytokine signaling in allergic inflammation, these results will help us to known signal controlled in CD4+ T lymphocytes and the mechanism of Chinese medicine.

Project ID:PC10008-0717
External Project ID:NSC100-2320-B182-032