Synthesis of Multi-Functional Nano-Magnetic Drugs and Application for Thrombolysis

The purpose of this project is to develop a material of magnetic nanoparticles (MNPs) that could carry the drugs for accelerating the dissolving of thrombolysis. One or two drugs such as thrombolytic drug (rt-PA), anticoagulants (Heparin), and antiplatelet drug (Tirofiban) are bound to the surfaces of MNPs and guided by the applied external magnet for concentration effective and targeted therapy. This is a 2 year project, which comprises the following research topics: 1. Synthesis the water solvable Fe3O4 nanoparticles with various sizes and characterize their structural and physical properties. 2. Synthesis the water suspension core/shell nanoparticles, Fe3O4/Poly(ethylene Oxide-acylic acid) (PEA) and Fe3O4/polyethylene oxide-polyacylic acid blend (PEAB). The above mentioned drugs can be bound on the surface of Fe3O4/PEA and Fe3O4/PEAB nanoparticles by covalent bonding and dispersed uniformly in water. Structural and physical properties of Fe3O4/PEA and Fe3O4/PEAB nanoparticles are characterized and correlated to their in-vitro therapy effect. 3. Activity analysis of Fe3O4, Fe3O4/PEA and Fe3O4/PEAB nanoparticles binding with drugs. 4. Cytotoxicity assay of Fe3O4, Fe3O4/PEA and Fe3O4/PEAB nanoparticles binding with drugs. 5. In-vitro thrombolysis of Fe3O4/PEA and Fe3O4/PEAB nanoparticles binding with drugs.

Project ID:PB9912-1073
External Project ID:NSC99-2221-E182-068