Synthysis of Carbon Quantum Dots for Ultrasensitive Immunoassay

Immunoassays are currently widely used in immunoassays such as enzyme-linked immunosorbent assay (ELISA) and Western blot. Horseradish peroxidase (horseradish peroxidase (HRP)) acts as a catalytic enzyme in the immunoassays. 3,3’,5,5’-tetramethylbenzidine (TMB) and luminol are common substrates for HRP-mediated catalytic reactions in the immunoassays. Even optical detection which mediated by HRP enzymes can meet most of the immunoassays, it could not detect ultralow concentrations of tumor markers, microRNA, and circulating tumor cells (CTCs) due to the low catalytic activity of HRP enzyme and the short lifetime and instability of intermediates. The aim of this project is to synthesize carbon quantum dots (CQDs) for improving the sensitivity of immunoassays. This project will employ the as-prepared CQDs as enhancers or substrates in immunoassays. CQDs can interact with HRP to alter its catalytic activity or enhance the stability of intermediates, thereby improving the detection limit of detection (LOD) of immunoassays, which can be used for ultrasensitive analysis of tumor markers, microRNA and CTCs in the future.

Project ID:PB10907-4421
External Project ID:MOST109-2622-E182-004-CC2