The Analgesic Effect of Pulsed Radiofrequency on Streptozotocin-Induced Diabetic Neuropathic Pain in Rats

  • Lin, Chung-Ren (PI)
  • Chen, Jiin-Tsuey (CoPI)

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details

Abstract

Patients with diabetes and neuropathy report a significantly decreased quality of life secondary to diabetic neuropathic pain (DNP), which is a major complication of diabetic neuropathy. Patients with DNP experience lower extremity burning or "shock-like sensations" with increased sensitivity to both painful (hyperalgesia) and nonpainful stimuli (allodynia). Despite the high morbidity of DNP, mechanisms underlying the onset and progression of this complication are poorly understood and the treatments are not satisfied. In this proposal, we plan to explore the therapeutic effect and underlying mechanism of pulsed radiofrequency (PRF) in DNP. To achieve this goal, we will first establish the diabetic painful neuropathy as an animal model of DNP. PRF will be then delivered to dorsal root ganglions (DRG) to examine the therapeutic profiles on DNP, including DRG applied (L5, L4+L5, and L3-5), intervention time points, and effective duration. To better understanding the underlying mechanisms or intracellular signaling pathway involved in the PRF on DNP model, neurotransmitters changes in CSF, neuronglial activation, MAPKs and their downstream gene expression profiles, their subcellular localization will be identified by intrathecal microdialysis, HPLC, RT-PCR, Western blot, and immunohistochemical study. Eletrophysiological study of spinal slices will also be performed to look at the change of descending inhibition pathway. To date, the application of PRF on DNP in human is still undetermined. Therefore, we believe that our study will disclose the underlying mechanism of PRF on DNP and provide an alternative therapeutic model for DNP.

Project IDs

Project ID:PC10401-0624
External Project ID:MOST103-2314-B182-045-MY3
StatusFinished
Effective start/end date01/08/1531/07/16

Keywords

  • pulsed radiofrequency
  • disinhibition
  • GABAergic
  • glycinergic
  • diabetic

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