Project Details
Abstract
Patients with diabetes and neuropathy report a significantly decreased quality of life
secondary to diabetic neuropathic pain (DNP), which is a major complication of diabetic
neuropathy. Patients with DNP experience lower extremity burning or "shock-like
sensations" with increased sensitivity to both painful (hyperalgesia) and nonpainful stimuli
(allodynia). Despite the high morbidity of DNP, mechanisms underlying the onset and
progression of this complication are poorly understood and the treatments are not satisfied.
In this proposal, we plan to explore the therapeutic effect and underlying mechanism of
pulsed radiofrequency (PRF) in DNP. To achieve this goal, we will first establish the
diabetic painful neuropathy as an animal model of DNP. PRF will be then delivered to dorsal
root ganglions (DRG) to examine the therapeutic profiles on DNP, including DRG applied
(L5, L4+L5, and L3-5), intervention time points, and effective duration. To better
understanding the underlying mechanisms or intracellular signaling pathway involved in the
PRF on DNP model, neurotransmitters changes in CSF, neuronglial activation, MAPKs and
their downstream gene expression profiles, their subcellular localization will be identified by
intrathecal microdialysis, HPLC, RT-PCR, Western blot, and immunohistochemical study.
Eletrophysiological study of spinal slices will also be performed to look at the change of
descending inhibition pathway.
To date, the application of PRF on DNP in human is still undetermined. Therefore, we
believe that our study will disclose the underlying mechanism of PRF on DNP and provide
an alternative therapeutic model for DNP.
Project IDs
Project ID:PC10401-0624
External Project ID:MOST103-2314-B182-045-MY3
External Project ID:MOST103-2314-B182-045-MY3
Status | Finished |
---|---|
Effective start/end date | 01/08/15 → 31/07/16 |
Keywords
- pulsed radiofrequency
- disinhibition
- GABAergic
- glycinergic
- diabetic
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