The Roles of Nucleophosmin/B23 in Cellular Mortalization

We have found previously that Nucleophosmin/B23 protein and mRNA level were both up-regulated quickly through an immediate-early response. A variety of inhibitors which could block specific kinase or transcriptional factor in pathways induced by UV irradiation were used to elucidate which pathway is important in UV induced Nucleophosmin/B23 up-regulation. These inhibitors were added to cells 30 min before UV irradiation, however, none of these inhibitors could block UV induced Nucleophosmin/B23 up-regulation. These results suggest that UV induces Nucleophosmin/B23 up-regulation through an unknown pathway. TT dimers, which mimic UV induced DNA fragementation, could up-regulate Nucleophosmin/B23 promoter activity and protein level without UV treatment. These findings will be helpful for the understandings of cells responsing to UV induced DNA damage.

Project ID:PG9303-1379
External Project ID:NHRI-EX93-8935SL