The Study of Establishment of a Label Free Cancer Stem Cell Selection System (II)

Cancer stem cells (CSCs) represent a subpopulation of tumor cells with self-renewal, tumor initiation, disease relapse or metastasis, and resistance to chemotherapy and radiotherapy capacities. However, the major obstacle in CSCs is the difficulty in their isolation and enrichment. According to current studies, CSCs shared the similar properties with normal stem cells and it is revealed that hyaluronan (HA) plays key factor in CSCs niches and HA-mediated CD44 interaction promotes tumor progression. Therefore, hyaluronan (HA) based multilayer films were used to fabricate sequential surface properties variation and to mimic the CSCs niches. Quartz crystal microbalance was used to investigate the layer by layer adsorption of PAH/ HA multilayer films. Colony formation was observed on series of poly (allylamine hydrochloride) PAH/HA multilayer films and the cell cytotoxicity and cell viability were evaluated by MTT, LDH and live/dead assay. It is revealed that the cells isolated from (PAH/ HA)3 displayed the best colony formation ability and the expression of CD133/ CD44 double positive cells was up-regulated to about 70% after 7 days culture. Furthermore, the cells isolated from (PAH/ HA)3 displayed high chemo-resistant property and high relative viability in comparison with control group. The stem cell related genes expression of selected cells from (PAH/ HA)3 after 7 days culture shows significant difference with control group. In conclusion, this study provides a label-free selection and enrichment system which could serve as a new strategy for the future development on CSCs selection and drug evaluation on cancer therapy.

Project ID:PB10308-2725
External Project ID:MOST103-2221-E182-011