The Study of Photoacoustic Effect of Liposome with Encapsulated GNPs)/GNRs (II)

This proposal, from Aug. 2014 to July 2016, is the extension of the grant: NSC 102-2221-E-182-049. It aims to study the photoacoustic (PA) effect of liposomes with encapsulated gold nanoparticles (GNPs), gold nanorods (GNRs) or coreshell GNRs (GNR@SiO2) irradiated by pulsed laser using the photoacoustic spectroscopy. The synthesis method (thin-film hydration method with sonication method) will be used to prepare the liposomes with encapsulated GNPs, GNRs or GNR@SiO2s. A filter (polycarbonate membrane) with a specific pore size will be used to control the size of liposome. In addition, the synthesized liposome-GNPs will be characterized by ICP-OES and UV-NIR spectrometer. The scattering and absorption behaviors of the aggregated GNPs/GNRs/GNR@SiO2s will also be studied theoretically. Using the dark-field microscopy, the morphologies of the cells internalizing liposomes with encapsulated GNPs/GNRs/GNR@SiO2s before and after the irradiation of pulsed laser will be analyzed. Moreover, the hyperspectral dark-field microscopy will be used to measure the scattering spectrum of each liposome-GNPs/GNRs/GNR@SiO2s. We have successfully synthesized the liposome-GNPs with size of 1 jam recently. According to the schedule, the synthesis of liposomes-GNRs and liposome-GNR@SiO2s with size smaller than 400 nm will be achieved at the July of 2015 and July of 2016, respectively. Recently, we are developing a photoacoustic spectroscopy using ultrasonic transducer to detect the PA signals emitted from the cavitation of the transient plasmonic nanobubbles (PNBs) around GNP, as the liposomes-GNPs are irradiated by pulsed laser. These transient PNBs around each GNP could damage the liposome. So far, the intensity of PA signal can be detected by a broadband ultrasonic transducer, but the measurement of lifetime of nanobubbles needs to be improved. For the liposome with GNRs, the tunable infrared wavelength of Nd: YAG laser with OPO will be used. The size of the liposome will be measured by dark-field microscopy, and the dose of GNPs will be measured quantitatively by ICP-OES. The cells cultured with a medium containing liposome with encapsulated GNPs will be prepared. The dose of GNPs/GNRs internalized by cells will be measured to compare with that of the control incubated with a GNPs-medium without liposome. After that, the photodamage of these cells irradiated by the pulsed laser will be studied. In addition, the behavior of laser induced microbubble (LIMB) in the colloid of liposome-GNPs/GNRs/GNR@SiO2s will also be investigated. The mechanism of LIMB is due to the optical dielectric breakdown at the focus in the medium, which is different from that of plasmonic nanobubbles.

Project ID:PB10401-1791
External Project ID:MOST103-2221-E182-033-MY2