The Use of Metabonomic Approach to Understand the Metabolic Disorders in Diabetic Rats and the Therapeutic Benefits of Resveratrol in Rats

Project: National Science and Technology CouncilNational Science and Technology Council Academic Grants

Project Details


Diabetes mellitus (DM) has become one of the main threats to human health in the 21st century. It is projected that by the year 2010, about 220 million people will be inflicted. Our understanding of the DM is largely on the detrimental effects of hyperglycemia and hyperlipidemia, including its pathophysiological effects on cardiovascular, renal and nervous systems. In recent years, research on the pathogenesis of DM and its complications have been focussed on AGE-RAGE elicited signals and their effects, increased fatty acid levels and insulin resistance, adipocytes and adipokines, increased ROS and inflammation in a systemic nature etc.. Thus, a systemic, in vivo approach to study the DM-inflicted metabolic disorders appeared to be appropriate. Here, we propose a relatively more holistic approach, the metabonomics, to detect, quantify, and catalogue the time related metabolic processes of the DM rats (with reference to the age-matched normal rats), and to relate such processes to the trajectories of the pathophysiological events occurring in DM , and to explore the action mechanisms underlying the metabolic benefits of resveratrol (a compound that has been shown to have metabolic benefits in mice on high fat diet, and in streptozotocin-induced DM rats). In recenty years, systemic oxidative stress and inflammation have been believed to implicate in DM pathology. Here are propose that the burdens of oxidative stress/inflammation could be varied among tissues/organs resulting in varied organ/tissue pathological complications in DM animals/subjects. In this proposal , we will also quantitate and track the changes of some selected biomarks for oxidative strss and in lammation in plasma and tissues/organs (including brain, liver, kidneys, heart, skeletal muscles and fat tissues etc.) of the normal and Dm rats with and without RSV treatment. We have performed some preliminary studies to analyse the urine metabolites of the normal, DM, and resveratrol-treated DM rats. Through the “condition tree” analysis, we have been able to see prominent differences among the urine metabolite complements of the 3 groups of rats. (part of our results have been published in American J. physiology, please see the Preliminary Results). Through our study, we should be able to gain better understanding of the pathophysiology of the DM rats and the health benefits of the resveratrol in the areas of energy metabolism.

Project IDs

Project ID:PC10208-0582
External Project ID:NSC102-2320-B182-033
Effective start/end date01/08/1331/07/14


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