同時偵測多種微小核酸之方法

The invention provides a method for detecting various micro nucleic acids at the same time, which comprises: first mixing the sample reagent under test, fluorescent probe and bridging nucleic acids of different lengths into a solution under test; during the process, allowing the hybridization of the probe and the nucleic acids under test with the bridging nucleic acids; then adding a ligase to carry out a ligation reaction between the short nucleic acid molecules of the reagent and the fluorescent probe by using the bridging nucleic acids as the template; then introducing the solution under test into a capillary and imposing an electric field to allow electrophoresis separation of the hybridized products; and finally exciting these products by laser and analyzing the fluorescent signals released from these products. Therefore, various short nucleic acid molecules in a sample under test can be detected simultaneously in a capillary.