氣功外氣對人類早期單核細胞株和流行性感冒病毒的效應

　　　　　介白質 6（ Interleukin 6 ）是調節發炎反應的一種細胞激素，主要是由單核白 血球及巨噬細胞系統之細胞所產生，它可以調節細胞的生長與分化，引起發炎反應並參與造 血過程，部份且具有抗病毒及抗腫瘤的作用，它可經由不同病毒，細胞激素或酯醣的刺激而 上升。 人類早期單核白血球細胞株（ HL-CZ ）可被流行性感冒病毒感染， 同時又屬於單核白 血球巨噬細胞系統的前身， 因此用來研究流行性感冒病毒對細胞激素介白質 6 基因表現。 本實驗是以 HL-CZ 先接受氣功師或氣功機發功（補氣或殺氣） 30 分鐘後， 接種低濃度流 行性感冒病毒 A/Taiwan/H1N1 株，以介白質 6 的傳訊核醣核酸（ mRNA ）之定量以反轉錄 酵素聚合酵素連鎖反應（ RT-PCR ）， 並以競爭性聚合酵素連鎖反應（ Competitive-PCR ）分別於不同的時間來看其量的變化。 同時並收集上清液以酵素免疫分析法（ ELISA ）測 介白質 6 的表現情況。 本文發現以低濃度的 A 型流行性感冒病毒並未能誘發介白質 6 的表現，而補氣或殺氣 都可以激活流行感冒病毒對 IL-6 的轉錄作用，補氣的效應明顯大於殺氣，但對轉譯則無此 效應而適當的溫度（ 37 ℃）可加強 IL-6 的轉錄效應過低或過高的溫度（ <37 ℃ or 50 ℃）則無此效應。但當和流行性感冒病毒同時作用則有協同 IL-6 轉錄的作用基本上氣可以 經由訊息的傳遞及基因的表現而造成細胞激素的改變，但意念和氣迄今仍無法作一有效的定 量，仍須進一步的研究及探討。
　　　　　Interleukin 6 is a kind of cytokines regulating inflammatory and immunological responses. It is usually produced by cells of monocyte/macrophage lineage. Their activities display a very similar but not identical spectrum including differentiation and growth-regulating of the cells, inflammatory, hematopoietic processes of the host sometimes with probably antiviral and antitumoral activities. A human promoncyte cell line, HL-CZ, has been established in our labaratory since 1985. It is of interest to see the cytokine activities in this cell line. Expression of IL-6 was detected in HL-CZ when the cells were infected with influenza/A/Taiwan/H1N1 at high multiplicity in our previous studies. The design in this study is to let HL-CZ cells received bioenergy from meditator or Qigong machine for 30 minutes, then infected with influenza/A/Taiwan/H1N1 at very low multiplicity (m.o.i=0.005). The mRNA level of IL-6 was analysed by reverse transcriptase polymerase chain reaction (RT-PCR) and then quantitated by competitive PCR. In this way, a small variation in mRNA level when amplified by RT-PCR could be quantitated by comparing with an internal standard of specific DNA. To investigate the mRNA expression, the level of IL-6 in the supernatant was detected by ELISA. In this study, we demonstrate that the influenza A virus in low multiplicity (m.o.i=0.005) is not capable of inducing IL-6 transcription. Both benificial Qi and destroying Qi can stimulate influenza A virus inducing IL-6 transcription but not translation. It is clear that benificial Qi is better than destroying Qi. The human temperature (about 37℃) can induce IL-6 transcription but not lower or higher temperature (37℃ or 50℃). But lower temperature can synchronize with influenza A virus for IL-6 transcription. Basically, this kind of energy (Qi) may cause biological change in the molecular level with signal transduction and gene expression. This bioenergy effect on the cells or viruses might go through a kind of psychokinesis. Unfortunately, psychokinesis and Qi can not be effectively quantified. The mechanism of Qi operation is of interest for further investigation.